PMEL17/SILV Antibody

Western Blot: PMEL17/SILV Antibody [NB100-41098] - Staining of Mouse Skin lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: PMEL17/SILV Antibody [NB100-41098] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PMEL17/SILV Antibody Summary

• Description: Novus Biologicals Goat PMEL17/SILV Antibody (NB100-41098) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-PMEL17/SILV Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CPIGENSPLLSGQQ corresponding to C-Terminus according to NP_008859.1.
• Marker: Melanosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PMEL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 26 kDa band observed in human skin lysates, corresponding to the M-beta fragment of the precursor protein, reported in scientific literature (PMID: 23389629). Calculated MW of 70.3 kDa band according to NP_008859.1
• Control:

  PMEL17/SILV Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24572365).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PMEL17/SILV Antibody

• D12S53EP1
• Gp100
• ME20
• ME20M
• ME20-M
• melanocyte protein mel 17
• Melanocyte protein Pmel 17
• Melanocytes lineage-specific antigen GP100
• Melanoma-associated ME20 antigen
• melanosomal matrix protein17
• PMEL
• PMEL17
• PMEL17P100
• premelanosome proteinME20M
• SI
• SIL
• SILV
• silver (mouse homolog) like
• silver homolog (mouse)
• Silver locus protein homolog
• silver, mouse, homolog of
• SILVPmel17

Background

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PMEL17/SILV Antibody (NB100-41098)(4)

Publications using NB100-41098

• Glodde N, Bald T, van den Boorn-Konijnenberg D et al. Reactive Neutrophil Responses Dependent on the Receptor Tyrosine Kinase c-MET Limit Cancer Immunotherapy. Immunity. 2017-10-17 [PMID: 29045907] (Mouse)
• Bald T, Quast T, Landsberg J et al. Ultraviolet-radiation-induced inflammation promotes angiotropism and metastasis in melanoma. Nature 2014-03-06 [PMID: 24572365] (WB, Mouse)
• Fowler DM, Koulov AV, Alory-Jost C et al. Functional amyloid formation within mammalian tissue. PLoS Biol 2006-01-01 [PMID: 16300414]
• Glodde N, Kraut A, van den Boorn-Konijnenberg D Experimental and stochastic models of melanoma T-cell therapy define impact of subclone fitness on selection of antigen loss variants bioRxiv (WB, Mouse)

Bioinformatics

• Gene Symbol: PMEL
• Entrez:
  • 6490(Human)
  • 20431(Mouse)
  • 362818(Rat)
• Uniprot:
  • P40967(Human)