PMEL17/SILV Antibody (SPM142) [Janelia Fluor® 585]

Product Discontinued

PMEL17/SILV Antibody (SPM142) [Janelia Fluor® 585] Summary

• Immunogen: Extract of pigmented melanoma metastases from lymph nodes
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. It stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This monoclonal antibody also stains Angiomyolipoma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMEL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Canine or Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PMEL17/SILV Antibody (SPM142) [Janelia Fluor® 585]

• D12S53EP1
• Gp100
• ME20
• ME20M
• ME20-M
• melanocyte protein mel 17
• Melanocyte protein Pmel 17
• Melanocytes lineage-specific antigen GP100
• Melanoma-associated ME20 antigen
• melanosomal matrix protein17
• PMEL
• PMEL17
• PMEL17P100
• premelanosome proteinME20M
• SI
• SIL
• SILV
• silver (mouse homolog) like
• silver homolog (mouse)
• Silver locus protein homolog
• silver, mouse, homolog of
• SILVPmel17

Background

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMEL