PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free

Western Blot: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779] - Western Blot Analysis of COLO-38 cell lysate using PMEL17/SILV Mouse Monoclonal Antibody (PMEL/783).

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779] - Human Testis stained with gp100 / Melanosome Monoclonal Antibody (PMEL/783).

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779] - Human Melanoma stained with gp100 / Melanosome Monoclonal Antibody (PMEL/783).

• Reactivity: Hu
• Applications: WB, IHC
• Clone: PMEL/783
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44522).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human PMEL17/SILV protein (Uniprot: P40967)
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMEL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Western Blot 2-4 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free

• D12S53EP1
• Gp100
• ME20
• ME20M
• ME20-M
• melanocyte protein mel 17
• Melanocyte protein Pmel 17
• Melanocytes lineage-specific antigen GP100
• Melanoma-associated ME20 antigen
• melanosomal matrix protein17
• PMEL
• PMEL17
• PMEL17P100
• premelanosome proteinME20M
• SI
• SIL
• SILV
• silver (mouse homolog) like
• silver homolog (mouse)
• Silver locus protein homolog
• silver, mouse, homolog of
• SILVPmel17

Background

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMEL