Human PLOD3 ELISA Kit (Colorimetric)

ELISA: Human PLOD3 ELISA Kit (Colorimetric) [NBP2-81259] - Samples were spiked with high concentrations of Human PLOD3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human PLOD3 ELISA Kit (Colorimetric) [NBP2-81259] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL

Human PLOD3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human PLOD3 in samples. No significant cross-reactivity or interference between Human PLOD3 and analogues was observed.
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.11%
• Intra-Assay: CV% < 4.87%
• Spike Recovery: 85-105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PLOD3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PLOD3 ELISA Kit (Colorimetric)

• 2-oxoglutarate 5-dioxygenase 3
• EC 1.14.11.4
• LH3Lysyl hydroxylase 3
• lysine hydroxylase 3
• procollagen-lysine
• procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3

Background

PLOD3 is encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PLOD3