Plexin C1 Antibody [Unconjugated]

Human whole blood monocytes were stained with Goat Anti-Human Plexin C1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3887, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Product Discontinued

Plexin C1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Plexin C1
  Ile49-Ser161
  Accession # O60486
• Specificity: Detects human Plexin C1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately
  10% cross‑reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A2, rmPlexin A3, and recombinant human (rh) Plexin B1 is observed, and less than 1% cross‑reactivity with rhPlexin D1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLXNC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin C1 Antibody [Unconjugated]

• CD232 antigen
• CD232
• plexin (semaphorin receptor)
• Plexin C1
• PLXNC1
• PLXN-C1
• receptor for viral semaphorin protein
• receptor for virally-encoded semaphorin
• VESPR
• VESPRplexin-C1
• Virus-encoded semaphorin protein receptor

Background

Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210 kDa type I transmembrane glycoprotein in the C subfamily of the Plexin family (1, 2). Human Plexin C1 contains a 34 amino acid (aa) signal sequence, a 910 aa extracellular domain (ECD) with one Sema-domain and two cysteine‑rich Met-related sequences (MRS), a 21 aa transmembrane domain and a 603 aa cytoplasmic domain that includes features common to other plexins, including a phosphothreonine site. The human Plexin C1 ECD shares 85%, 82% and 71% aa identity with murine, bovine and opossum Plexin C1, respectively. Plexin C1 is widely expressed in neuronal and non-neuronal fetal and adult tissues (3). In neuronal development, its role is unclear. Semaphorin-7a (Sema 7A, CD108) binds Plexin C1 in vitro, and the two show a similar expression pattern during rat neuronal development. However, in rat, beta 1 integrins rather than Plexin C1 appear to mediate Sema 7A effects on axon outgrowth (4, 5). Plexin C1 does appear to play a role in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, as indicated by specific defects seen in mice deleted for Plexin C1 (6). In the immune system, effects of Sema 7A on T cell-mediated inflammatory responses also appear to be mediated by beta 1 integrins rather than plexins (7). However, Plexin C1 may function to oppose the effect of beta 1 integrins, as it does on Sema 7A-mediated spreading and dendrite formation in melanocytes (8). Plexin C1 is the receptor for the poxvirus (A39R protein) and herpes virus (AHVsema) semaphorin homologs, and mediates activation of monocytes and inhibition of dendritic cell and neutrophil phagocytosis by A39R (2, 9, 10).

1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363. 
2. Comeau, M.R. et al. (1998) Immunity 8:473. 
3. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355. 
4. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98. 
5. Pasterkamp, R.J. et al. (2003) Nature 424:398. 
6. Xu, C. and C-M. Fan (2007) Mol. Endocrinol. 21:1234.
7. Suzuki, K. et al. (2007) Nature 446:680.
8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
9. Walzer, T. et al. (2005) J. Immunol. 174:51.
10. Walzer, T. et al. (2005) Eur. J. Immunol. 35:391.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plexin C1 Antibody (AF3887)(2)

Publications using AF3887

• L Man, N Lustgarten, E Kallinos, Z Redhead-La, S Liu, B Schattman, D Redmond, K Hancock, N Zaninovic, G Schattman, Z Rosenwaks, D James Comparison of Human Antral Follicles of Xenograft versus Ovarian Origin Reveals Disparate Molecular Signatures Cell Rep, 2020-08-11;32(6):108027. 2020-08-11 [PMID: 32783948] (Flow Cytometry, ICC, IHC, Human)
• Morote-Garcia J, Napiwotzky D, Kohler D, Rosenberger P Endothelial Semaphorin 7A promotes neutrophil migration during hypoxia. Proc Natl Acad Sci U S A, 2012-08-13;109(35):14146-51. 2012-08-13 [PMID: 22891341] (Blocking, Human)

Bioinformatics

• Gene Symbol: PLXNC1
• Uniprot:
  • O60486()