Plexin B2 Antibody (537237) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 537237
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Plexin B2 Antibody (537237) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Plexin B2
  Leu20-Arg1160
  Accession # O15031
• Specificity: Detects human Plexin B2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Plexin A4, B1, B3, C1, D1, or recombinant mouse Plexin A1, A2, or A3 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLXNB2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin B2 Antibody (537237) [Unconjugated]

• MM1
• MM1KIAA0315dJ402G11.3
• Nbla00445
• PLEXB2
• Plexin B2
• plexin-B2
• PLXNB2

Background

Plexin B2 is a 240 kDa member of the B subfamily, plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes, and serves as a receptor for Semaphorin 4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178 aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20‑466) and three IPT repeats (aa 803‑1092). The ECD may be cleaved into two subunits, a 170 kDa alpha -chain (aa 20‑1164) and an 80 kDa TM beta ‑chain that remain noncovalently‑linked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa 67‑638. Over aa 20‑1160, human Plexin B2 is 82% aa identical to mouse Plexin B2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plexin B2 Antibody (MAB5329)(2)

Publications using MAB5329

• EE Evans, V Mishra, C Mallow, EM Gersz, L Balch, A Howell, C Reilly, ES Smith, TL Fisher, M Zauderer Semaphorin 4D is upregulated in neurons of diseased brains and triggers astrocyte reactivity Journal of Neuroinflammation, 2022-08-06;19(1):200. 2022-08-06 [PMID: 35933420] (Functional Assay, Mouse)
• Meda C, Molla F, De Pizzol M Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages. J. Immunol., 2012-03-21;188(8):4081-92. 2012-03-21 [PMID: 22442441] (Neutralization, Human)

Bioinformatics

• Gene Symbol: PLXNB2
• Uniprot:
  • O15031()