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Plexin B1 Antibody (559830) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
559830
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 405
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB37491U-100UG
Alexa Fluor 488
FAB37491G-100UG
Alexa Fluor 594
FAB37491T-100UG
Alexa Fluor 647
FAB37491R-100UG
Alexa Fluor 700
FAB37491N-100UG
Alexa Fluor 750
FAB37491S-100UG
Allophycocyanin
FAB37491A
Phycoerythrin
FAB37491P
Unconjugated
MAB37491
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB37491
Carrier Free
MAB37491
Carrier Free
FAB37491P
Carrier Free
MAB37491-SP
Carrier Free
FAB37491A
Carrier Free
FAB37491U-100UG
Carrier Free
FAB37491T-100UG
Carrier Free
FAB37491S-100UG
Carrier Free
FAB37491R-100UG
Carrier Free
FAB37491N-100UG
Carrier Free
FAB37491G-100UG
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Plexin B1 Antibody (559830) [Alexa Fluor® 405] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Plexin B1
Accession # O43157
Specificity
Detects human Plexin B1 in direct ELISAs.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLXNB1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin B1 Antibody (559830) [Alexa Fluor® 405]

  • Plexin B1
  • PLXN5
  • PLXNB1
  • SEP
  • Transmembrane Protein SEP

Background

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with neuropilins, MET, and EGF R2. Of three known splice variants, one shows a deletion of aa 689-871, a second shows a 53 aa substitution for aa 677‑2135, and a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 shares 90% and 97% aa sequence identity with dog and mouse Plexin B1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN.
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Exosome component
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Antibody [Unconjugate ...
AF1034
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Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cathepsin C/DPPI at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cathepsin C/DPPI was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). Specific staining was localized to thymocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(1 Review)
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Applications: WB, Simple Western, IHC
NOC2L Antibody
NBP1-92190
Western Blot: NOC2L Antibody [NBP1-92190] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/Immunofluorescence: NOC2L Antibody [NBP1-92190] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
Immunohistochemistry-Paraffin: NOC2L Antibody [NBP1-92190] - Staining of human cerebellum shows strong nucleolar positivity in Purkinje cells.
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Applications: WB, ICC/IF, IHC, IHC-P, KD

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB37491U
Alexa Fluor 405 FAB37491V
Alexa Fluor 488 FAB37491G
Alexa Fluor 594 FAB37491T
Alexa Fluor 647 FAB37491R
Alexa Fluor 700 FAB37491N
Alexa Fluor 750 FAB37491S
Allophycocyanin FAB37491A
Phycoerythrin FAB37491P
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol PLXNB1
Uniprot