PLEKHH1 Antibody (3-E11)

Western Blot: PLEKHH1 Antibody (3-E11) [NBP2-75622] - Analysis of PLEKHH1 on different cell lysates using anti-PLEKHH1 antibody at 1/100 dilution.Positive control:Lane 1: MCF-7 Lane 2: A431Lane 3: SH-SY5Y

Immunocytochemistry/Immunofluorescence: PLEKHH1 Antibody (3-E11) [NBP2-75622] - Staining PLEKHH1 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PLEKHH1 Antibody (3-E11) [NBP2-75622] - Analysis of paraffin-embedded mouse kidney tissue using anti-PLEKHH1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PLEKHH1 Antibody (3-E11) [NBP2-75622] - Analysis of paraffin-embedded human kidney tissue using anti-PLEKHH1 antibody. Counter stained with hematoxylin.

Product Discontinued

PLEKHH1 Antibody (3-E11) Summary

• Immunogen: Recombinant protein within Human PLEKHH1 aa 1,059-1,364 / 1,364. (SwissProt: Q9ULM0 Human)
• Localization: Cytoskeleton.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLEKHH1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 151 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for PLEKHH1 Antibody (3-E11)

• PLEKHH1 pleckstrin homology domain containing, family H (with MyTH4 domain) member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLEKHH1