PLC-gamma 2 [p Tyr753] Antibody (790623) [Unconjugated]

Western blot shows lysates of RL human non-Hodgkin's lymphoma B cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-PLC- gamma2 (Y753) Monoclonal Antibody (Catalog # MAB37161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-PLC- gamma2 (Y753) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 790623
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PLC-gamma 2 [p Tyr753] Antibody (790623) [Unconjugated] Summary

• Immunogen: Phosphopeptide containing the human PLC‑ gamma 2 Y753 site
  Accession # P16885
• Modification: p Tyr753
• Specificity: Detects human PLC‑ gamma 2 when phosphorylated at Y753.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLCG2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLC-gamma 2 [p Tyr753] Antibody (790623) [Unconjugated]

• EC 3.1.4.11
• Phosphoinositide phospholipase C-gamma-2
• phospholipase C gamma 2
• phospholipase C, gamma 2 (phosphatidylinositol-specific)
• phospholipase C, gamma 2 (phosphatidylyinositol-specific)
• Phospholipase C-gamma-2,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2
• Phospholipase C-IV
• PLCG2
• PLCgamma 2
• PLC-gamma 2
• PLC-gamma-2
• PLC-IV

Background

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta , -gamma , -epsilon , -zeta , and -eta . PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones, and downstream through Lck kinase-dependent phosphorylation at Y753 and Y759. Activated PLC-gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes. Human PLC-gamma 2 shares 94% and 95% sequence identity overall with mouse and rat PLC-gamma 2, respectively, and 100% sequence identity within the peptide immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCG2
• Uniprot:
  • P16885()