PLC-gamma 2 Antibody (744757) [Alexa Fluor® 350]

• Applications: WB, ICC/IF
• Clone: 744757
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

PLC-gamma 2 Antibody (744757) [Alexa Fluor® 350] Summary

• Immunogen: Phosphopeptide containing the human PLC-γ2 Y759 site
  Accession # P16885
• Specificity: Detects human Phospho-PLC-gamma 2 (Y759) in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta , -gamma , -epsilon , -zeta , and -eta . PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones, and downstream through Lck kinase-dependent phosphorylation at Y753 and Y759. Activated PLC-gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes. Human PLC-gamma 2 shares 94% and 95% sequence identity overall with mouse and rat PLC-gamma 2, respectively, and 100% sequence identity within the peptide immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.