PLC-gamma 2 Antibody (346404)

Western blot shows lysates of A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLC- gamma2 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PLC- gamma2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 346404
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PLC-gamma 2 Antibody (346404) Summary

• Immunogen: E. coli-derived recombinant human PLC-gamma 2
  Pro400-Tyr600
  Accession # P16885
• Specificity: Detects human and mouse PLC-gamma 2.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLCG2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLC-gamma 2 Antibody (346404)

• EC 3.1.4.11
• Phosphoinositide phospholipase C-gamma-2
• phospholipase C gamma 2
• phospholipase C, gamma 2 (phosphatidylinositol-specific)
• phospholipase C, gamma 2 (phosphatidylyinositol-specific)
• Phospholipase C-gamma-2,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2
• Phospholipase C-IV
• PLCG2
• PLCgamma 2
• PLC-gamma 2
• PLC-gamma-2
• PLC-IV

Background

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta , -gamma , -epsilon , -zeta , and -eta . PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-  gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones. Activated PLC-  gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-  gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLC-gamma 2 Antibody (MAB3716)(1)

Publication using MAB3716

• A Al Subayyi, YS Basmaeil, R Alenzi, T Khatlani Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist-induced platelet functions reducing atherosclerosis and thrombosis phenotypes Journal of Cellular and Molecular Medicine, 2021-09-18;0(0):. 9/18/2021 [PMID: 34535958]

Bioinformatics

• Gene Symbol: PLCG2
• Uniprot:
  • P16885()