PLC-beta 3 Antibody (JE39-14)

Western Blot: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Western blot analysis of PLC-beta 3 on different lysates with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HCT 116 cell lysate Lane 5: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32776) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32776) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: JE39-14
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PLC-beta 3 Antibody (JE39-14) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human PLC-beta 3 aa 1,161-1,210 / 1,234. (Uniprot: Q01970)
• Localization: Cytoplasm. Membrane. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLCB3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:1000
• Theoretical MW: 139 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PLC-beta 3 Antibody (JE39-14)

• 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3
• EC 3.1.4.11
• FLJ37084
• Phosphoinositide phospholipase C-beta-3
• phospholipase C, beta 3 (phosphatidylinositol-specific)
• Phospholipase C-beta-3
• PLC beta 3
• PLCB3
• PLCbeta 3
• PLC-beta 3
• PLC-beta-3

Background

Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5 bisphosphate. A total of eight mammalian PLC isozymes have been described (PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC gamma1, PLC gamma2, PLC delta1 and PLC delta2) with molecular weights ranging from 85 to 150 kDa. The gamma-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two gamma-type enzymes, but not the beta and delta isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC beta1, PLC beta2 and PLC beta3 is mediated by the alpha subunits of the Gq class of heterotrimeric G proteins and by certain betagamma G protein subunits. The regulatory mechanisms for PLC delta1 and PLC delta2 are as yet not resolved.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCB3