PLC-beta 1 Antibody

Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 1 was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 1 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PLC-beta 1 Antibody Summary

• Immunogen: E. coli-derived recombinant human PLC-beta 1
  Lys27-Met245
  Accession # Q9NQ66
• Specificity: Detects human and mouse PLC-beta 1, but does not detect recombinant human (rh) PLC-beta 2, rhPLC-beta 3, or rhPLC-beta 4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PLCB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLC-beta 1 Antibody

• EC 3.1.4.11
• EIEE12
• FLJ45792
• inositoltrisphosphohydrolase
• KIAA05811-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 1
• monophosphatidylinositol phosphodiesterase
• phosphoinositidase C
• Phosphoinositide phospholipase C-beta-1
• phospholipase C, beta 1 (phosphoinositide-specific)
• Phospholipase C-beta-1
• Phospholipase C-I
• PI-PLC
• PLC-154
• PLC154,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1
• PLCB1
• PLCB1A
• PLCB1B
• PLCbeta 1
• PLC-beta 1
• PLC-beta-1
• PLC-I
• PLC-I1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
• triphosphoinositide phosphodiesterase

Background

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta , -gamma , -epsilon , -zeta , and -eta . PLC-beta 1 (Phospholipase C beta-1) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4, 5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. Alternative splicing results in two transcript variants encoding two isoforms, PLC-beta 1A and PLC-beta 1B. PLC-beta 1A is preferentially expressed in the cytosol, while PLC-beta 1B is predominantly expressed in the nucleus. The region used as immunogen is 100% identical in both isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCB1
• Uniprot:
  • Q9NQ66()