Mouse Plasminogen ELISA Kit (Colorimetric)

ELISA: Mouse Plasminogen ELISA Kit (Colorimetric) [NBP2-60521] - Standard curve.

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Cell Culture, Urine
• Standard Curve Range: 0.094 - 6 ng/mL (example only; lot dependent)
• Sensitivity: 0.14 ng/ml (example only; lot dependent)

Mouse Plasminogen ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.094 - 6 ng/mL (example only; lot dependent)
• Sensitivity: 0.14 ng/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.50% (example only; lot dependent)
• Intra-Assay: CV% < 5.00% (example only; lot dependent)
• Spike Recovery: 97.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PLG

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Plasminogen ELISA Kit (Colorimetric)

• DKFZp779M0222
• EC 3.4.21
• EC 3.4.21.7
• plasmin
• Plasminogen
• Plg

Background

Plasminogen is a plasma protein synthesised mostly in the liver. Plasma concentration is usually in the range of 70 to 200mg/L. Plasminogen is activated by tissue plasminogen activator, urokinase and streptokinase, to form plasmin. Activation results from the cleavage and release of the preactivation peptide. Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Plasminogen ELISA Kit (NBP2-60521). (Showing 1 - 2 of 2 FAQ).

1. What is the difference between your Human Plasminogen ELISA kits NBP2-60521, NBP2-60515 and NBP2-61297? Also, have these been tested for reactivity against plasmin?
   • Our Human Plasminogen ELISA kits NBP2-60521, NBP2-60515 and NBP2-61297 are all colorimeteric ELISA kits. However, these kits are different in reference to the suitable sample type, sensitivity, detection range etc. NBP2-61297 is a Competitive ELISA kit while the other two are Sandwich ELISA based. Also, NBP2-60521 is a mouse reactive kit while the other two are good for human samples. 
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PLG