Plakophilin 1 Antibody (10B2) - Azide and BSA Free

Western Blot: Plakophilin 1 Antibody (10B2) - Azide and BSA Free [NBP2-54526] - Western Blot of U87, HeLa and A431 cell lysates using Plakophilin 1 Antibody (10B2)

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: 10B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Plakophilin 1 Antibody (10B2) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53326).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: N-terminus of human Plakophilin 1 protein (Uniprot: Q13835)
• Epitope: aa 1-27
• Localization: Cell Surface and nuclear
• Specificity: Recognizes a protein of 75kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PKP1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Western Blot 0.5 - 1.0 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Plakophilin 1 Antibody (10B2) - Azide and BSA Free

• B6PBand 6 protein
• MGC138829
• plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
• plakophilin-1

Background

The Plakophilin 1 gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PKP1