Rat PLA2G7/PAF-AH/Lp-PLA2 ELISA Kit (Colorimetric)

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ELISA: Rat PLA2G7/PAF-AH/Lp-PLA2 ELISA Kit (Colorimetric) [NBP2-81153] - Samples were spiked with high concentrations of Rat PLA2G7/PAF-AH/Lp-PLA2 and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Rat PLA2G7/PAF-AH/Lp-PLA2 ELISA Kit (Colorimetric) [NBP2-81153] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
7.81 - 500 ng/mL
Sensitivity
4.69 ng/mL

Order Details

Rat PLA2G7/PAF-AH/Lp-PLA2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat PLA2G7/PAF-AH/Lp-PLA2 in samples. No significant cross-reactivity or interference between Rat PLA2G7/PAF-AH/Lp-PLA2 and analogues was observed.
Standard Curve Range
7.81 - 500 ng/mL
Sensitivity
4.69 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.29%
Intra-Assay
CV% < 5.6%
Spike Recovery
87-106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PLA2G7

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat PLA2G7/PAF-AH/Lp-PLA2 ELISA Kit (Colorimetric)

  • 2-acetyl-1-alkylglycerophosphocholine esterase
  • EC 3.1.1
  • EC 3.1.1.47,1-alkyl-2-acetylglycerophosphocholine esterase
  • Group-VIIA phospholipase A2
  • gVIIA-PLA2
  • LDL-associated phospholipase A2
  • LDL-PLA(2)
  • LDL-PLA2
  • lipoprotein-associated phospholipase A2
  • LpPLA2
  • Lp-PLA2
  • PAF acetylhydrolase
  • PAF-AH
  • PAFAHPAF 2-acylhydrolase
  • phospholipase A2, group VII (platelet-activating factor acetylhydrolase
  • PLA2G7
  • plasma)
  • platelet-activating factor acetylhydrolase

Background

The protein encoded by the PLA2G7 gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PLA2G7