PLA2G7/PAF-AH/Lp-PLA2 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: CoraFluor 1

PLA2G7/PAF-AH/Lp-PLA2 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human PLA2G7/PAF-AH/Lp-PLA2
  Met33-Asn441
  Accession # AAH38452
• Specificity: Detects human PLA2G7/PAF-AH/Lp-PLA2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PLA2G-2A, recombinant mouse PLA2G-2A, and rhPLA2G-1B is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLA2G7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PLA2G7/PAF-AH/Lp-PLA2 Antibody [CoraFluor™ 1]

• 2-acetyl-1-alkylglycerophosphocholine esterase
• EC 3.1.1
• EC 3.1.1.47,1-alkyl-2-acetylglycerophosphocholine esterase
• Group-VIIA phospholipase A2
• gVIIA-PLA2
• LDL-associated phospholipase A2
• LDL-PLA(2)
• LDL-PLA2
• lipoprotein-associated phospholipase A2
• LpPLA2
• Lp-PLA2
• PAF acetylhydrolase
• PAF-AH
• PAFAHPAF 2-acylhydrolase
• phospholipase A2, group VII (platelet-activating factor acetylhydrolase
• PLA2G7
• plasma)
• platelet-activating factor acetylhydrolase

Background

The protein encoded by the PLA2G7 gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLA2G7