PKC epsilon Antibody [Alexa Fluor® 532]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

PKC epsilon Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human PKCε
  Gln580-Pro737
  Accession # Q02156
• Specificity: Detects human, mouse and rat PKC epsilon in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKC epsilon Antibody [Alexa Fluor® 532]

• EC 2.7.11
• EC 2.7.11.13
• MGC125656
• nPKC-epsilon
• PKC epsilon
• PKCE
• PKCEMGC125657
• PRKCE
• protein kinase C epsilon type
• protein kinase C, epsilon

Background

PKC epsilon (protein kinase C-epsilon) is an 87 kDa member of the novel PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a widely-expressed Ca++‑insensitive, phospholipid-dependent enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC epsilon is 737 amino acids (aa) in length. It contains two general regions: a non-Ca++-binding plus lipid binding regulatory region (aa’s 1‑99 and 169‑292, respectively), and an ATP-binding catalytic domain (aa 408‑668). Phosphorylation of PKC epsilon on Thr566, Ser368 and Ser729 activates its enzymatic activity and increases its molecular weight to 92 kDa. One potential splice form shows a 114 aa substitution for aa 118‑737. Over aa 580‑737, human PKC epsilon is 98% and 99% aa identical to mouse and canine PKC epsilon , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.