PKC epsilon Antibody (JA38-21)

Western Blot: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Analysis of PKC epsilon on different cell lysates using anti-PKC epsilon antibody at 1/1,000 dilution. Positive control:Lane1: Jurkat Lane2: MCF-7 Lane3: HT-29

Immunocytochemistry/Immunofluorescence: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Staining PKC epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Analysis of paraffin-embedded rat brain tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.

Flow Cytometry: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Flow cytometric analysis of PKC epsilon was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Staining PKC epsilon in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Analysis of paraffin-embedded human kidney tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Analysis of paraffin-embedded human pancreas tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Analysis of paraffin-embedded mouse testis tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.

Product Discontinued

PKC epsilon Antibody (JA38-21) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PKC epsilon aa 561-610 / 737. (SwissProt: Q02156 Human; SwissProt: P16054 Mouse; SwissProt: P09216 Rat)
• Localization: Cytoplasm.?Membrane.?Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PKC epsilon Antibody (JA38-21)

• EC 2.7.11
• EC 2.7.11.13
• MGC125656
• nPKC-epsilon
• PKC epsilon
• PKCE
• PKCEMGC125657
• PRKCE
• protein kinase C epsilon type
• protein kinase C, epsilon

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCE