PKC epsilon Antibody (666823) [Alexa Fluor® 488]

• Applications: IHC
• Clone: 666823
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

PKC epsilon Antibody (666823) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PKCε
  Gln580-Pro737
  Accession # Q02156
• Specificity: Detects human PKC epsilon in direct ELISAs. In direct ELISAs, less than 20% cross-reactivity with recombinant human (rh) PKCδ and rhPKC iota is observed. No cross-reactivity with rhPKC alpha , beta 1, beta 2, gamma , eta , μ, nu , theta , or zeta is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

PKC epsilon (protein kinase C-epsilon) is an 87 kDa member of the novel PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a widely-expressed Ca⁺⁺‑insensitive, phospholipid-dependent enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC epsilon is 737 amino acids (aa) in length. It contains two general regions: a non-Ca⁺⁺-binding plus lipid binding regulatory region (aa 1-99 and 169-292, respectively), and an ATP-binding catalytic domain (aa 408-668). Phosphorylation of PKC epsilon on Thr566, Ser368 and Ser729 activates the enzyme and increases its molecular weight to 92 kDa. One potential splice form shows a 114 aa substitution for aa 118-737. Over aa 580-737, human PKC epsilon is 98% and 99% aa identical to mouse and canine PKC epsilon , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.