Recombinant Human PKC alpha Protein

Product Discontinued

Recombinant Human PKC alpha Protein Summary

• Description: Recombinant protein for Human PKC alpha
  
  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(P17252)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab60839)
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: PRKCA
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 69 nmol phosphate incorporated into CREBtide per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PKC alpha Protein

• AAG6
• aging-associated gene 6
• EC 2.7.11
• EC 2.7.11.13
• MGC129901
• PKC alpha
• PKCA
• PKC-A
• PKC-alpha
• PKCAMGC129900
• PRKACA
• PRKCA
• protein kinase C alpha type
• protein kinase C, alpha

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Coussens defined a new family of PKC-related genes and termedalpha, Beta , and gamma (1). Latos-Bielenska refined the assignment of PRKCA1 to 17q22-q23.2 (2). Braz identified PKC-alpha as a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes in study of knockout mice (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for PKC alpha Recombinant Protein (NBP1-99883). (Showing 1 - 1 of 1 FAQ).

1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
   • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

Bioinformatics

• Gene Symbol: PRKCA