PKC alpha Antibody (D22-G) [FITC]

Images

 
Flow Cytometry: PKC alpha Antibody (D22-G) [FITC] [NBP2-21819] - Profile of peripheral blood lymphocytes. Cells were fixed, permeabilized and stained with anti-human PKCalpha FITC (blue, used 10 ul per test) or with an ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
D22-G
Clonality
Monoclonal
Host
Rabbit
Conjugate
FITC

Order Details

PKC alpha Antibody (D22-G) [FITC] Summary

Immunogen
This antibody targets PKC alpha.
Clonality
Monoclonal
Host
Rabbit
Gene
PRKCA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100

Reactivity Notes

Human, Mouse and Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris/HCI (pH 8.0) and 10 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for PKC alpha Antibody (D22-G) [FITC]

  • AAG6
  • aging-associated gene 6
  • EC 2.7.11
  • EC 2.7.11.13
  • MGC129901
  • PKC alpha
  • PKCA
  • PKC-A
  • PKC-alpha
  • PKCAMGC129900
  • PRKACA
  • PRKCA
  • protein kinase C alpha type
  • protein kinase C, alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for PKC alpha Antibody (NBP2-21819). (Showing 1 - 1 of 1 FAQ).

  1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
    • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

Secondary Antibodies

 

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Blogs on PKC alpha.

There is nothing beta than PKC Alpha
cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely rel...  Read full blog post.

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Bioinformatics

Gene Symbol PRKCA
Entrez