Recombinant Human PKA C-gamma Protein

HPLC: Recombinant PKA C-gamma Protein [NBP1-99511]

Product Discontinued

Recombinant Human PKA C-gamma Protein Summary

• Description: Recombinant protein for Human PKA C-gamma
  
  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NM_002732)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab60838)
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: PRKACG
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: The specific activity of PKAcg was determined to be 145 nmol /min/mg as per activity assay protocol.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PKA C-gamma Protein

• cAMP-dependent protein kinase catalytic subunit gamma
• EC 2.7.11
• EC 2.7.11.11
• KAPG
• PKA C-gamma
• PKACg
• PRKACG
• protein kinase, cAMP-dependent, catalytic, gamma
• serine(threonine) protein kinase

Background

PKA C-gamma (PKAcg) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C-alpha and C-Beta and showed tissue-specific expression. Whereas at the amino acid level C-alpha and C-Beta showed 93% homology, C-gamma showed only about 80% homology to both C-alpha and C-Beta (1). The PRKACG gene is intronless, contains remnants of a poly (A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene(2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PRKACG