Recombinant Human PKA C-gamma Protein

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HPLC: Recombinant PKA C-gamma Protein [NBP1-99511]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.1 mg/ml

Order Details

Recombinant Human PKA C-gamma Protein Summary

Description
Recombinant protein for Human PKA C-gamma

Source:Baculovirus (Sf9 insect cells)
Amino Acid Sequence:(NM_002732)

Source
Sf 9 (baculovirus)
Protein/Peptide Type
Biologically Active Protein
Gene
PRKACG
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Specific activity: The specific activity of PKAcg was determined to be 145 nmol /min/mg as per activity assay protocol.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol
Concentration
0.1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PKA C-gamma Protein

  • cAMP-dependent protein kinase catalytic subunit gamma
  • EC 2.7.11
  • EC 2.7.11.11
  • KAPG
  • PKA C-gamma
  • PKACg
  • protein kinase, cAMP-dependent, catalytic, gamma
  • serine(threonine) protein kinase

Background

PKA C-gamma (PKAcg) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C-alpha and C-Beta and showed tissue-specific expression. Whereas at the amino acid level C-alpha and C-Beta showed 93% homology, C-gamma showed only about 80% homology to both C-alpha and C-Beta (1). The PRKACG gene is intronless, contains remnants of a poly (A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene(2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol PRKACG