Recombinant Human PKA C beta Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.1 mg/ml

Order Details

Recombinant Human PKA C beta Protein Summary

Description
Recombinant protein for Human PKA C beta

Source:Baculovirus (Sf9 insect cells)
Amino Acid Sequence:(NM_002731)

Source
Sf 9 (baculovirus)
Protein/Peptide Type
Biologically Active Protein
Gene
PRKACB
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Specific activity: 342 umol phosphate incorporated into CREBtide substrate per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Concentration
0.1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PKA C beta Protein

  • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
  • cAMP-dependent protein kinase catalytic subunit beta
  • cAPKbeta
  • DKFZp781I2452
  • EC 2.7.11
  • EC 2.7.11.11
  • MGC41879
  • MGC9320
  • PKA C beta
  • PKA C-beta
  • PKACb
  • PRKACB
  • protein kinase A catalytic subunit beta
  • protein kinase, cAMP-dependent, catalytic, beta

Background

Most of the effects of cAMP are mediated through the phosphorylation of target proteins on serine or threonine residues by the cAMP-dependent protein kinase (PKA). The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. The mammalian catalytic subunit has been shown to consist of three PKA gene products: C-alpha, C-Beta, and C-gamma. Two PKA isoforms exist, designated types I and II, which differ in their dimeric regulatory subunits, designated RI and RII, respectively. Furthermore, there are at least four different regulatory subunits: RI-alpha, RI-Beta, RII-alpha, and RII-Beta. The cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The catalytic subunit C-Beta of PKA (PKAcb) is a member of the Ser/Thr protein kinase family and is a catalytic subunit C-Beta of AMPK. Berube et al. assigned the PKAcb to human chromosome 1 by Southern blot analysis of somatic cell hybrids (1) and Simard et al located it to 1p36.1 by in situ hybridization (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol PRKACB
Entrez