Pirh2 Antibody (1H10)

Images

 
Western Blot: Pirh2 Antibody (1H10) [NBP1-51604] - Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Pirh2 Antibody (1H10) [NBP1-51604] - Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 ...read more
Immunohistochemistry-Paraffin: Pirh2 Antibody (1H10) [NBP1-51604] - Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb.
Flow Cytometry: Pirh2 Antibody (1H10) [NBP1-51604] - Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-P
Clone
1H10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Pirh2 Antibody (1H10) Summary

Immunogen
Purified recombinant fragment of human Pirh2 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RCHY1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry-Paraffin
Application Notes
This product is specific for a homodimer of two 30kDa proteins, so it may show as 60kDa on SDS page

Reactivity Notes

Human, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Alternate Names for Pirh2 Antibody (1H10)

  • Androgen receptor N-terminal-interacting protein
  • androgen-receptor N-terminal-interacting protein
  • ARNIPRING finger protein 199
  • CHIMPRNF199DKFZp586C1620
  • CH-rich interacting match with PLAG1
  • CH-rich-interacting match with PLAG1
  • E3 ubiquitin-protein ligase Pirh2
  • EC 6.3.2.-
  • hARNIP
  • hPirh2
  • PIRH2
  • PRO1996
  • ring finger and CHY zinc finger domain containing 1
  • RING finger and CHY zinc finger domain-containing protein 1
  • Zinc finger protein 363p53-induced RING-H2 protein
  • ZNF363

Background

The protein encoded by this gene has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pirh2 Antibody (NBP1-51604) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RCHY1