Pirh2 Antibody (1H10)

Western Blot: Pirh2 Antibody (1H10) [NBP1-51604] - Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.

Immunocytochemistry/Immunofluorescence: Pirh2 Antibody (1H10) [NBP1-51604] - Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Pirh2 Antibody (1H10) [NBP1-51604] - Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb.

Flow Cytometry: Pirh2 Antibody (1H10) [NBP1-51604] - Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).

Product Discontinued

Pirh2 Antibody (1H10) Summary

• Immunogen: Purified recombinant fragment of human Pirh2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RCHY1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This product is specific for a homodimer of two 30kDa proteins, so it may show as 60kDa on SDS page

Reactivity Notes

Human, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Pirh2 Antibody (1H10)

• Androgen receptor N-terminal-interacting protein
• androgen-receptor N-terminal-interacting protein
• ARNIPRING finger protein 199
• CHIMPRNF199DKFZp586C1620
• CH-rich interacting match with PLAG1
• CH-rich-interacting match with PLAG1
• E3 ubiquitin-protein ligase Pirh2
• EC 6.3.2.-
• hARNIP
• hPirh2
• PIRH2
• PRO1996
• ring finger and CHY zinc finger domain containing 1
• RING finger and CHY zinc finger domain-containing protein 1
• Zinc finger protein 363p53-induced RING-H2 protein
• ZNF363

Background

The protein encoded by this gene has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RCHY1