PIP5K2 alpha Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

PIP5K2 alpha Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human PIP5K2 alpha.
  
  

  Source: E. coli

  Amino Acid Sequence: NDFINEGQKIYIDDNNKKVF

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: PIP4K2A
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-49566.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for PIP5K2 alpha Recombinant Protein Antigen

• 1-phosphatidylinositol-4-phosphate kinase
• 1-phosphatidylinositol-4-phosphate-5-kinase
• 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha
• Diphosphoinositide kinase 2-alpha
• EC 2.7.1
• EC 2.7.1.149
• FLJ13267
• phosphatidylinositol-4-phosphate 5-kinase, type II, alpha
• Phosphatidylinositol-5-phosphate 4-kinase type II alpha
• phosphatidylinositol-5-phosphate 4-kinase type-2 alpha
• phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• PI(5)P 4-kinase type II alpha
• PIP4KII-alpha
• PIP5K2
• PIP5K2API5P4KA
• PIP5KIIA
• PIP5KIIalpha
• PIP5KII-alpha
• PIP5KIII
• PIPK
• PtdIns(4)P-5-kinase B isoform
• PtdIns(4)P-5-kinase C isoform
• PtdIns(5)P-4-kinase isoform 2-alpha
• type II phosphatidylinositol-4-phosphate 5-kinase 53 K isoform

Background

Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PIP4K2A