PIP5K2 alpha Knockout HeLa Cell Lysate

Product Discontinued

PIP5K2 alpha Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon1 and Insertion of the selection cassette in exon1
• Gene: PIP4K2A

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for PIP5K2 alpha Knockout HeLa Cell Lysate

• 1-phosphatidylinositol-4-phosphate kinase
• 1-phosphatidylinositol-4-phosphate-5-kinase
• 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha
• Diphosphoinositide kinase 2-alpha
• EC 2.7.1
• EC 2.7.1.149
• FLJ13267
• phosphatidylinositol-4-phosphate 5-kinase, type II, alpha
• Phosphatidylinositol-5-phosphate 4-kinase type II alpha
• phosphatidylinositol-5-phosphate 4-kinase type-2 alpha
• phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• PI(5)P 4-kinase type II alpha
• PIP4KII-alpha
• PIP5K2
• PIP5K2API5P4KA
• PIP5KIIA
• PIP5KIIalpha
• PIP5KII-alpha
• PIP5KIII
• PIPK
• PtdIns(4)P-5-kinase B isoform
• PtdIns(4)P-5-kinase C isoform
• PtdIns(5)P-4-kinase isoform 2-alpha
• type II phosphatidylinositol-4-phosphate 5-kinase 53 K isoform

Background

Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PIP4K2A