Recombinant Human PIP His Protein

SDS-Page: Recombinant Human PIP Protein [NBP2-23366]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human PIP His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 29-146 of Human PIP
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSQDNTRKI IIKNFDIPKS VRPNDEVTAV LAVQTELKEC MVVKTYLISS IPLQGAFNYK YTACLCDDNP KTFYWDFYTN RTVQIAAVVD VIRELGICPD DAAVIPIKNN RFYTIEILKV E

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PIP
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 15.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PIP His Protein

• GCDFP15prolactin-inducible protein
• GCDFP-15SABP
• GPIP4gp17
• Gross cystic disease fluid protein 15
• prolactin-induced proteinSecretory actin-binding protein

Background

Prolactin-inducible protein, also known as PIP, is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. It is expressed in exocrine glands and, in pathologic conditions, in breast cysts and breast cancers exhibiting apocrine features. PIP and prostate specific antigen are co-expressed in androgen receptor-positive breast tumours. Recombinant human PIP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PIP