PIP Antibody

Western Blot: PIP Antibody [NB100-2923] - (0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PIP Antibody [NB100-2923] - (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PIP Antibody Summary

• Immunogen: Peptide with sequence C-GANKAQDNTRK corresponding to internal region according to NP_002643.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PIP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 14 kDa band observed in A431 lysates (calculated MW of 16.6 kDa band according to NP_002643.1). IHC-P: Human breast shows staining of luminal epithelial cells of the lactiferous duct.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIP Antibody

• GCDFP15prolactin-inducible protein
• GCDFP-15SABP
• GPIP4gp17
• Gross cystic disease fluid protein 15
• prolactin-induced proteinSecretory actin-binding protein

Background

Gross cystic disease of the breast is benign premenopausal disorder in which cysts are a predominant pathological lesion. These cysts appear to be formed from excessive aporcine cystic secretions. This fluid is composed of several glycoproteins including a unique 15kD monomer protein, PIP. Cytosolic analysis of normal tissue specimens from all major organs has demonstrated PIP in aporcine epithelia, lacrimal, ceruminous and Mollus glands and in numerous serous cells of the submandibular, tracheal, bronchial, sublingual and minor salivary glands. PIP and prostate specific antigen are co-expressed in androgen receptor-positive breast tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIP Antibody (NB100-2923)(1)

Publication using NB100-2923

• O'Connell FP, Wang HH, Odze RD. Utility of immunohistochemistry in distinguishing primary adenocarcinomas from metastatic breast carcinomas in the gastrointestinal tract. Arch Pathol Lab Med 2005-03-01 [PMID: 15737028]

Bioinformatics

• Gene Symbol: PIP
• Entrez:
  • 5304(Human)
• Uniprot:
  • NP_002643.1(Human)