PIK3R5 Antibody (OTI4G9)

Western Blot: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3R5.

Immunocytochemistry/Immunofluorescence: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PIK3R5.

Immunohistochemistry: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI4G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PIK3R5 Antibody (OTI4G9) Summary

• Immunogen: Full length human recombinant protein of human PIK3R5(NP_055123) produced in HEk293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIK3R5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 97.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIK3R5 Antibody (OTI4G9)

• F730038I15Rik
• FOAP-2
• p101
• p101-PI3K
• Phosphatidylinositol-4,5-bisphosphate 3-kinase regulatory subunit
• phosphoinositide-3-kinase, regulatory subunit 5
• phosphoinositide-3-kinase, regulatory subunit, polypeptide p101
• PI3-kinase p101 subunit
• PI3-kinase regulatory subunit 5
• PIK3R5
• PtdIns-3-kinase regulatory subunit
• regulatory subunit 5, p101

Background

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of 85 kDa (p85) and 110 kDa (p110) subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85alpha and p85beta), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110alpha and p110beta interact with p85alpha, and p110alpha has also been shown to interact with p85beta in vitro. p110delta expression is restricted to white blood cells. It has been shown to bind p85alpha and beta, but it apparently does not phosphorylate these subunits. p110delta seems to have the capacity to autophosphorylate. p110gamma does not interact with the p85 subunits. It has been shown to be activated by alpha and betagamma heterotrimeric G proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIK3R5 Antibody (NBP2-46413)(1)

Publication using NBP2-46413

• Li L, Kim H, Park S et al. Genetic Profiles Associated with Chemoresistance in Patient-Derived Xenograft Models of Ovarian Cancer. Cancer Res Treat. 2018-11-06 [PMID: 30428638] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: PIK3R5