Human PIK3C2A - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human PIK3C2A - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39341] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates and other biological fluids
• Standard Curve Range: 3.12 - 200 U/L (example only; lot dependent)
• Sensitivity: 1.6 U/L (example only; lot dependent)

Human PIK3C2A - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 3.12 - 200 U/L (example only; lot dependent)
• Sensitivity: 1.6 U/L (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PIK3C2A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PIK3C2A - Ready-To-Use ELISA Kit (Colorimetric)

• C2-containing phosphatidylinositol kinase
• CPK
• DKFZp686L193
• EC 2.7.1
• EC 2.7.1.137
• EC 2.7.1.153
• EC 2.7.1.154
• MGC142218
• phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha
• Phosphoinositide 3-kinase-C2-alpha
• phosphoinositide-3-kinase, class 2, alpha polypeptide
• PI3-K-C2(ALPHA)
• PI3-K-C2A
• PI3K-C2alpha
• PI3K-C2-alpha
• PIK3C2A
• PtdIns-3-kinase C2 subunit alpha

Background

PIK3C2A belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PIK3C2A ELISA Kit (NBP3-39341). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PIK3C2A