PIK3C2A Antibody

Western Blot: PIK3C2A Antibody [NBP2-19829] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PIK3C2A Antibody [NBP2-19829] - Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using PI3 kinase C2 alpha antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:500.

Immunohistochemistry-Paraffin: PIK3C2A Antibody [NBP2-19829] - Paraffin-embedded hepatoma. PI3K C2 alpha antibody [C3], C-term dilution: 1:200.

Product Discontinued

PIK3C2A Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PI3KC2A. The exact sequence is proprietary.
• Localization: Cell membrane; Golgi apparatus; Cytoplasmic vesicle; clathrin-coated vesicle; Nucleus; Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIK3C2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Application Notes: Knockdown data (PMID: 31941925).
• Theoretical MW: 191 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31941925).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PIK3C2A Antibody

• C2-containing phosphatidylinositol kinase
• CPK
• DKFZp686L193
• EC 2.7.1
• EC 2.7.1.137
• EC 2.7.1.153
• EC 2.7.1.154
• MGC142218
• phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha
• Phosphoinositide 3-kinase-C2-alpha
• phosphoinositide-3-kinase, class 2, alpha polypeptide
• PI3-K-C2(ALPHA)
• PI3-K-C2A
• PI3K-C2alpha
• PI3K-C2-alpha
• PIK3C2A
• PtdIns-3-kinase C2 subunit alpha

Background

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PIK3C2A Antibody (NBP2-19829)(1)

Publication using NBP2-19829

• Boukhalfa A, Nascimbeni AC, Ramel D et al. PI3KC2 alpha-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress Nat Commun 2020-01-15 [PMID: 31941925] (WB, KD, ICC/IF, Mouse, Human)

Bioinformatics

• Gene Symbol: PIK3C2A
• Uniprot:
  • O00443(Human)