PIK3C2A Antibody

Images

 
Western Blot: PIK3C2A Antibody [NBP2-19829] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PIK3C2A Antibody [NBP2-19829] - Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using PI3 kinase C2 alpha antibody (Green) at 1:500 dilution. Alpha-tubulin ...read more
Immunohistochemistry-Paraffin: PIK3C2A Antibody [NBP2-19829] - Sample: Paraffin-embedded hepatoma.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.8 mg/ml

Order Details

PIK3C2A Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PI3K C2 alpha. The exact sequence is proprietary.
Localization
Cell membrane; Golgi apparatus; Cytoplasmic vesicle; clathrin-coated vesicle; Nucleus; Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIK3C2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
191 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.8 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIK3C2A Antibody

  • C2-containing phosphatidylinositol kinase
  • CPK
  • DKFZp686L193
  • EC 2.7.1
  • EC 2.7.1.154
  • MGC142218
  • phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha
  • Phosphoinositide 3-kinase-C2-alpha
  • phosphoinositide-3-kinase, class 2, alpha polypeptide
  • PI3-K-C2(ALPHA)
  • PI3-K-C2A
  • PI3K-C2alpha
  • PI3K-C2-alpha
  • PtdIns-3-kinase C2 subunit alpha

Background

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PIK3C2A
Uniprot

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