pIgR Antibody (SC05) - Azide and BSA Free

Immunohistochemistry-Paraffin: pIgR Antibody (SC05/COMPO2) - Azide and BSA Free [NBP2-47138] - Venules in the interalveolar stroma are positive for the pIgR antigen. Image from a verified customer review. Antigen retrieval by a microwave method in 0.01M citric acid (pH 6.0). Alex Fluor 488 anti-mouse antibody was used as a secondary detection antibody.

Immunohistochemistry-Paraffin: pIgR Antibody (SC05) - Azide and BSA Free [NBP2-47138] - Formalin-fixed, paraffin-embedded human Colon Carcinoma.

Product Discontinued

pIgR Antibody (SC05) - Azide and BSA Free Summary

• Immunogen: Secretory Component protein isolated from human colostrum
• Localization: Cell surface and cytoplasmic
• Specificity: Reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIGR
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-2ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: IHC-P: Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Suggested dilution: 1-2ug/ml for 30 minutes at RT.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported in a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for pIgR Antibody (SC05) - Azide and BSA Free

• FLJ22667
• Free Secretory Component (FSC)
• hepatocellular carcinoma associated protein TB6
• Hepatocellular carcinoma-associated protein TB6
• MGC125361
• MGC125362
• pIgA-Secretory Component Complex
• pIgM-Secretory Component Complex
• pIgR
• Poly-Ig receptor
• polymeric immunoglobulin receptor
• Secretory Component (SC)
• sIgA-Secretory Component Complex

Background

The PIGR gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical sur

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for pIgR Antibody (NBP2-47138) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Porcine.

reviewed by: Verified Customer IHC-P Porcine 06/19/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mammary gland tissue
• Species: Porcine
• Lot: 1893

Comments

• Comments: Antigen retrieval by a microwave method in 0.01M citric acid (pH 6.0). Alex Fluor 488 anti-mouse antibody was used as a secondary detection antibody.

Bioinformatics

• Gene Symbol: PIGR
• Entrez:
  • 5284(Human)
• Uniprot:
  • P01833(Human)