PIEZO2 expression and mechanical characterization of MTN neurons in Piezo2cKO mice. Coronal brainstem sections of MTN neurons with DAPI stained nuclei (blue) that were immunolabeled for PIEZO2 (red, left) or GFP (green, ...read more
Analysis of PIEZO2 in dorsal root ganglion of embryonic day (E) 13.5 mouse. PIEZO2 in red, DAPI in blue. Antigen retrieval with 1x DAKO antigen retrieval solution, by heating in a microwave oven, for total time of 6 ...read more
Immunocytochemistry/ Immunofluorescence: PIEZO2 Antibody [NBP1-78624] - PIEZO2 antibody was tested at 1:50 in A431 cells with FITC (green) .Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Analysis of PIEZ02 in mouse epidermis using DAB with hematoxylin counterstain.
Analysis of a FFPE tissue section of human breast using 1:200 dilution of PIEZO2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were ...read more
Immunocytochemistry/ Immunofluorescence: PIEZO2 Antibody - BSA Free [NBP1-78624] - Suppression of MA in MTN neurons from adult C57BL/6J mice by silencing Piezo2.(a) Schematic representation of the unilateral ...read more
Western Blot 2 ug/ml. Use reported in scientific literature (PMID 31791130)
Application Notes
In ICC/IF, membrane staining was observed in A431 cells. In paraffin sections, membrane and cytoplasmic staining is seen in mouse epidermis tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Use in Rat reported in scientific literature (PMID:34335288) Guinea Pig reactivity reported in scientific literature (PMID: 30324494).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for PIEZO2 Antibody - BSA Free
C18orf30
C18orf58
DA3
DA5
DAIPT
FAM38B
FAM38B2
family with sequence similarity 38, member A pseud
family with sequence similarity 38, member B
family with sequence similarity 38, member B2
FLJ23144
FLJ23403
FLJ25916
FLJ34907
FLJ37734
FLJ45725
HsT748
HsT771
MWKS
PIEZO2
piezo-type mechanosensitive ion channel component
piezo-type mechanosensitive ion channel component 2
protein PIEZO2
Background
Piezo2 (FAM38B) is a mechanosensitive ion channel which responds to mechanically activated currents in bladder, colon, lung, and dorsal root ganglia. This suggests Piezo2 is important for somatasensory mechanotransduction including touch and pain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins, and are very large in size.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Gao S, Tang Y, Sun W et al. 3D-bioprinted GelMA nerve guidance conduits promoted peripheral nerve regeneration by inducing trans-differentiation of MSCs into SCLCs via PIEZO1/YAP axis Materials Today Advances 2023-03-01 (WB, Rat)
Jia X, Liu X, Zhu T et al. Infiltrated Macrophages Aggravate TMJOA Chronic Pain Via Piezo2 in IB4 -TG Neurons papers.ssrn.com 2023-11-28 (IHC-Fr, ICC/IF, Rat)
Duan M, Jia Y, Huo L et al. Potentiation of PIEZO2 mechanically-activated currents in sensory neurons mediates vincristine-induced mechanical hypersensitivity Acta Pharmaceutica Sinica B 2023-05-01 (Immunohistochemistry, Rat)
Antigen retrieval with 1x DAKO antigen retrieval solution, by heating in a microwave oven, for total time of 6 minutes 10 seconds. (0.01M citric acid, pH 6.0, can also be used.) Washing buffer 1 x PBS with 0.1% Triton X-100. Blocking in 5% donkey serum (Jackson lab) in washing buffer. Primary antibody diluted 1:1000, incubation 18 hours in room temperature. Secondary antibody donkey anti-rabbit Cy3 (Jackson lab), 1:400, 3 hours in room temperature.
Product General Protocols
View specific protocols for PIEZO2 Antibody (NBP1-78624):
FAQs for PIEZO2 Antibody (NBP1-78624). (Showing 1 - 2 of 2 FAQs).
In my project we need Anti Piezo2 and we find it in your catalogs. PIEZO2 Antibody. I want to know the prediction size of your antibody. The size of the protein is more than 200 kd. but in this photo there there are some sharp band are there the actual bands?
The predicted molecular weights for this protein are based on the multiple transcripts associated with this protein. Please see <a href=" http://may2012.archive.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000154864;r=18:10670238-11148761" target="_blank">Ensembl </a> for detailed information. Those expected molecular weights are the following: 318.1, 80.8, 73.8, 62.7 kDa. We see this kind of staining across many of our antibodies for PIEZO2. It would appear that the transcript present is dependent on the tissue type. Based on protein arrays, we do believe that this antibody is specific.
I am interested in purchasing the PIEZO 1 and PIEZO 2 antibodies and their blocking peptides from Novus. I have read your data sheets that in WBs the bands seen are approximately 286 kDa and 318 kDa, respectively. I would greatly appreciate if you could show me these WBs on human cells For PIEZO1 and 2 and the effects of the blocking peptides (or point me to publications using your Abs).
We offer a PIEZO1 blocking peptide specific to product NBP1-78446. We unfortunately do not offer a PIEZO2 blocking peptide. Our western blot data for NBP1-78446, NBP1-78624, and NBP1-78538 was performed by an outside collaborator and they have not given permission to us to release their images. That is why we do not have them available on our website. We will fully guarantee all of our products for any listed application or species. We unfortunately do not have any publications for these products either.
Touch Infographic: From Touch Receptors to the Brain The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for ... Read full blog post.
PIEZO1: A Mechanosensitive Ion Channel Protein PIEZO1, and its close homologue PIEZO2, are mechanosensitive ion channel proteins. Mechanosensitive ion channels couple protein conformation to the mechanics of the surrounding membrane, and switch between a closed state and an open state in response ... Read full blog post.
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![Immunocytochemistry/Immunofluorescence: PIEZO2 Antibody [NBP1-78624] - PIEZO2 antibody was tested at 1:50 in A431 cells with FITC (green) .Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/PIEZO2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78624-img0006.jpg "Immunocytochemistry/Immunofluorescence: PIEZO2 Antibody [NBP1-78624] - PIEZO2 antibody was tested at 1:50 in A431 cells with FITC (green) .Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunocytochemistry/ Immunofluorescence: PIEZO2 Antibody - BSA Free [NBP1-78624] - Suppression of MA in MTN neurons from adult C57BL/6J mice by silencing Piezo2.(a) Schematic representation of the unilateral adeno-associated viral (AAV) injection site targeting MTN neurons. (b) Fluorescence images showing the expression of GFP-tagged MTN neurons in brain slices following AAV delivery. (b’) Higher magnification of the boxed area in (b). (c) The AAV-Piezo2-sh-1 construct. (d) Brainstem coronal sections of C57BL/6J mice infected with the AVV-shRNA against Piezo2 (middle) & the scrambled AVV (lower), showing MTN neurons immunolabeled for Piezo2 (left, red) or GFP (middle, green), & the merged image of both, with the nuclei stained with DAPI (blue). Arrowheads indicate representative MTN neurons. Scale bar 50 μm. (e) Traces of MA currents evoked by mechanical indentation in MTN neurons infected with AAV-Piezo2-sh-1 or with a scrambled AVV (f). (g) Trace from a MTN neuron infected with AAV-Piezo2-sh-1 that responded with RA mechanical activated currents ( tau = 6.2 ms). The inset shows a higher magnification of the current trace corresponding to a 7 μm displacement. The black upper trace shows the 250 ms mechanical stimulus steps in 1 μm increments applied every 10 s, the breaks in the trace corresponding to equivalent breaks in the recording. (h) Histogram summarizing the mean amplitude of the RA currents in MTN neurons from WT mice (n = 69) & those infected with AAV-Piezo2-sh-1 (n = 20) or AAV-shScr (n = 8). The data are expressed as the means ± s.e.m.: *p < 0.05 (Student’s t-test). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep25923), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78624_rabbit-polyclonal-piezo2-antibody-31020241616027.jpg "Immunocytochemistry/ Immunofluorescence: PIEZO2 Antibody - BSA Free [NBP1-78624] - Suppression of MA in MTN neurons from adult C57BL/6J mice by silencing Piezo2.(a) Schematic representation of the unilateral adeno-associated viral (AAV) injection site targeting MTN neurons. (b) Fluorescence images showing the expression of GFP-tagged MTN neurons in brain slices following AAV delivery. (b’) Higher magnification of the boxed area in (b). (c) The AAV-Piezo2-sh-1 construct. (d) Brainstem coronal sections of C57BL/6J mice infected with the AVV-shRNA against Piezo2 (middle) & the scrambled AVV (lower), showing MTN neurons immunolabeled for Piezo2 (left, red) or GFP (middle, green), & the merged image of both, with the nuclei stained with DAPI (blue). Arrowheads indicate representative MTN neurons. Scale bar 50 μm. (e) Traces of MA currents evoked by mechanical indentation in MTN neurons infected with AAV-Piezo2-sh-1 or with a scrambled AVV (f). (g) Trace from a MTN neuron infected with AAV-Piezo2-sh-1 that responded with RA mechanical activated currents ( tau = 6.2 ms). The inset shows a higher magnification of the current trace corresponding to a 7 μm displacement. The black upper trace shows the 250 ms mechanical stimulus steps in 1 μm increments applied every 10 s, the breaks in the trace corresponding to equivalent breaks in the recording. (h) Histogram summarizing the mean amplitude of the RA currents in MTN neurons from WT mice (n = 69) & those infected with AAV-Piezo2-sh-1 (n = 20) or AAV-shScr (n = 8). The data are expressed as the means ± s.e.m.: *p < 0.05 (Student’s t-test). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep25923), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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