PIEZO1 Antibody (1081418) [Unconjugated]

PIEZO1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human PIEZO1 Monoclonal Antibody (Catalog # MAB11557) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 1081418
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PIEZO1 Antibody (1081418) [Unconjugated] Summary

• Immunogen: PIEZO1 containing synthetic peptide
  Accession # Q92508
• Specificity: Detects a synthetic peptide around aa 150 in Direct ELISA
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIEZO1 Antibody (1081418) [Unconjugated]

• FAM38A
• KIAA0233
• member A
• membrane protein induced by beta-amyloid treatment
• Mib
• PIEZO1
• piezo-type mechanosensitive ion channel component 1
• Protein FAM38A

Background

Piezo1 is an 287kDa mechanosensitive nonselective cation channel, extensively distributed in multiple tissues and cell types.  Piezo1 plays an important role in numerus physiological processes, such as determining vascular structure, urine osmoregulation, and blood pressure homeostasis.  In the kidney, Piezo1 expression is found in the renal corpuscle, convoluted tubules, and collecting ducts.

1. Zhao X, Kong Y, Liang B, Xu J, Lin Y, Zhou N, Li J, Jiang B, Cheng J, Li C, Wang W. Mechanosensitive Piezo1 channels mediate renal fibrosis. JCI Insight. 2022 Apr 8;7(7):e152330. doi: 10.1172/jci.insight.152330. PMID: 35230979; PMCID: PMC9057604.
2. Carrisoza-Gaytan R, Kroll KT, Hiratsuka K, Gupta NR, Morizane R, Lewis JA, Satlin LM. Functional maturation of kidney organoid tubules: PIEZO1-mediated Ca2+ signaling. Am J Physiol Cell Physiol. 2023 Mar 1;324(3):C757-C768. doi: 10.1152/ajpcell.00288.2022. Epub 2023 Feb 6. PMID: 36745528; PMCID: PMC10027089.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q92508(Human)