PIDD Antibody (Anto-1) - BSA Free

Western Blot: LRDD Antibody (Anto-1) [NBP1-97595] - Analysis of endogenous and overexpressed human PIDD using MAb to PIDD (Anto-1). Method: Cell extracts (1x10^5) from HEK 293T wild type (lane 1) or transfected with a plasmid coding for human PIDD (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to PIDD (Anto-1) at a 1:500 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. Note that two major forms of PIDD are detected, representing full length and cleaved proteins, migrating at approximatively 90kDa and 50kDa, respectively.

Immunohistochemistry-Paraffin: LRDD Antibody (Anto-1) [NBP1-97595] - Staining of endogenous human PIDD in different human tissues (paraffin sections) using MAb to PIDD (Anto-1). Method: Different human normal (A: Bronchus; B: Kidney; C: Lung) or cancer (D: Breast; E: Ovarian; F: Colo-rectal) tissues were stained with MAb to PIDD (Anto-1) by standard immunohistochemistry.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: Anto-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PIDD Antibody (Anto-1) - BSA Free Summary

• Immunogen: Recombinant human PIDD death domain (aa 776-910).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIDD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Detects bands of ~90kDa (full length protein) and ~50kDa (cleaved protein) by Western blot.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PIDD Antibody (Anto-1) - BSA Free

• leucine-rich repeat and death domain-containing protein
• leucine-rich repeats and death domain containing
• MGC16925
• p53-induced protein with a death domain
• PIDD

Background

The protein encoded by the LRDD gene contains a leucine-rich repeat and a death domain. This protein has been shown to interact with other death domain proteins, such as Fas (TNFRSF6)-associated via death domain (FADD) and MAP-kinase activating death domain-containing protein (MADD), and thus may function as an adaptor protein in cell death-related signaling processes. The expression of the mouse counterpart of this gene has been found to be positively regulated by the tumor suppressor p53 and to induce cell apoptosis in response to DNA damage, which suggests a role for this gene as an effector of p53-dependent apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been reported. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIDD Antibody (NBP1-97595)(4)

Publications using NBP1-97595

• Zhao Q, Hu J, Kong L et al. FGL2-targeting T cells exhibit antitumor effects on glioblastoma and recruit tumor-specific brain-resident memory T cells Nature communications 2023-02-10 [PMID: 36759517] (WB, Mouse)
• Liu PH, Shah RB, Li Y et al. An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy Nat. Cell Biol. 2019-02-01 [PMID: 30664786] (WB, Human)
• Kung CP, Khaku S, Jennis M et al. Identification of TRIML2, a Novel p53 Target, that Enhances p53-SUMOylation and Regulates the Transactivation of Pro-apoptotic Genes. Mol. Cancer Res. 2014-09-25 [PMID: 25256710] (WB, Human)
  
  Details:
  LRDD/PIDD antibody (Anto-1) used for WB on lysates of U2OS cells containing doxycycline-inducible vector alone or doxycycline-inducible TRIML2 following treatment with 0.75 ?g/mL doxycycline and 100 ?M etoposide for 24 hours (Figure 4D).
• A Tinel et al. Autoproteolysis of PIDD marks the bifurcation between pro-death caspase-2 and pro-survival NF-kappaB pathway. EMBO J. 26, 197. 2007-01-01 [PMID: 17159900]

Bioinformatics

• Gene Symbol: PIDD1