PIAS2 Antibody

Images

 
Western Blot: PIAS2 Antibody [NBP2-19819] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: PIAS2 Antibody [NBP2-19819] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Western Blot: PIAS2 Antibody [NBP2-19819] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PIAS2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human PIAS2. The exact sequence is proprietary.
Localization
Nucleus speckle
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIAS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-19819 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for PIAS2 Antibody

  • Androgen receptor-interacting protein 3
  • ARIP3
  • DAB2-interacting protein
  • DIP
  • E3 SUMO-protein ligase PIAS2
  • MGC102682
  • miz
  • MIZ1
  • Msx-interacting zinc finger protein
  • Msx-interacting-zinc finger
  • PIAS2
  • PIAS-NY protein
  • PIASx alpha
  • PIASX
  • PIASX-ALPHA
  • PIASX-BETA
  • Protein inhibitor of activated STAT x
  • protein inhibitor of activated STAT, 2
  • Protein inhibitor of activated STAT2
  • SIZ2
  • zinc finger, MIZ-type containing 4
  • ZMIZ4

Background

This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB2005
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP1-90214
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56603
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89150
Species: Hu, Rt
Applications: WB, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
NBP1-77158
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-41211
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, IF
NBP1-69571
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-57531
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-38530
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-87581
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-19819
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PIAS2 Antibody (NBP2-19819) (0)

There are no publications for PIAS2 Antibody (NBP2-19819).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for PIAS2 Antibody (NBP2-19819) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-19819:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PIAS2 NBP2-19819
Enlarge
reviewed by:
Anonymous
WB Human 09/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedTHP1 cells
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBST

Primary Anitbody

Dilution Ratio1:1K in PBST buffer

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit IgG Antibody (HRP)-Novus
Secondary Manufacturer Cat#Cat No NB7160
Secondary ConcentrationDiluted 20,000 times

Details

Detection NotesDetected using PromoKine ECL Chemiluminescence Detection Kit with a 1 min exposure on X-ray film

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PIAS2 Antibody (NBP2-19819) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional PIAS2 Products

Bioinformatics Tool for PIAS2 Antibody (NBP2-19819)

Discover related pathways, diseases and genes to PIAS2 Antibody (NBP2-19819). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PIAS2 Antibody (NBP2-19819)

Discover more about diseases related to PIAS2 Antibody (NBP2-19819).
 

Pathways for PIAS2 Antibody (NBP2-19819)

View related products by pathway.

PTMs for PIAS2 Antibody (NBP2-19819)

Learn more about PTMs related to PIAS2 Antibody (NBP2-19819).

Blogs on PIAS2

There are no specific blogs for PIAS2, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
09/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PIAS2