PIAS2 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

PIAS2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PIAS2
  Met1-Gly550
  Accession # O75928
• Specificity: Detects human PIAS2 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIAS2 Antibody [Alexa Fluor® 488]

• Androgen receptor-interacting protein 3
• ARIP3
• DAB2-interacting protein
• DIP
• E3 SUMO-protein ligase PIAS2
• MGC102682
• miz
• MIZ1
• Msx-interacting zinc finger protein
• Msx-interacting-zinc finger
• PIAS2
• PIAS-NY protein
• PIASx alpha
• PIASX
• PIASX-ALPHA
• PIASX-BETA
• Protein inhibitor of activated STAT x
• protein inhibitor of activated STAT, 2
• Protein inhibitor of activated STAT2
• SIZ2
• zinc finger, MIZ-type containing 4
• ZMIZ4

Background

PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx-beta ) is a 45‑70 kDa member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it is widely expressed in cultured cell lines. PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK‑1, promoting its activation via HDAC2 dissociation and desumoylation. Human PIAS2 is 621 amino acids (aa) in length. It contains a SAP domain (aa 11‑45), a zinc-finger region (aa 331‑408), a SUMO-binding motif (aa 467‑472) and an NLS (aa 484‑492). There are multiple splice variants. PIAS2x-beta /ARIP3 shows a 22 aa substitution for aa 551‑621, while PIAS-NY shows a 12 aa substitution for aa 1‑8, followed by a truncation after Gly401. Two others show a four aa substitution for aa 504‑621, and an alternate start site at Met10. Over aa 1‑550, human PIAS2 shares 98% aa identity with mouse PIAS2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.