PI4K2A Antibody - Azide and BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PI4K2A Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: PI4K2A Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide at the N-terminal of human P4K2A protein. (Uniprot: Q9BTU6)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PI4K2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry
  • Western Blot 1ug/ml
• Application Notes: This product has been tested by ELISA, and western blot and is useful for Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~54kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with human, mouse, and rat based on 100% sequence homology. Cross-reactivity with PI4KII alpha from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for PI4K2A Antibody - Azide and BSA Free

• DKFZp761G1923
• EC 2.7.1.67
• phosphatidylinositol 4-kinase type 2 alpha
• phosphatidylinositol 4-kinase type 2-alpha
• phosphatidylinositol 4-kinase type II (PI4KII)
• Phosphatidylinositol 4-kinase type II-alpha
• PI4KII
• PIK42A
• RP11-548K23.6

Background

Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PI4K2A
• Uniprot:
  • Q9BTU6(Human)