PI 3-Kinase p85 beta Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

PI 3-Kinase p85 beta Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human PI 3-Kinase p85 beta.

  Source: E. coli

  Amino Acid Sequence: EMQRILLNSERLKSRIAEIHESRTKLEQQLRAQASDN

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: PIK3R2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-56360. It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml. For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for PI 3-Kinase p85 beta Recombinant Protein Antigen

• P85B
• p85-beta
• phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
• phosphatidylinositol 3-kinase regulatory subunit beta
• phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta)
• phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• PI 3Kinase p85 beta
• PI 3-Kinase p85 beta
• PI3K regulatory subunit beta
• PI3-kinase regulatory subunit beta
• PI3-kinase subunit p85-beta
• PIK3R2
• ptdIns-3-kinase regulatory subunit beta
• ptdIns-3-kinase regulatory subunit p85-beta

Background

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 beta). p85 beta is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 beta is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PIK3R2