PI 3-Kinase p85 beta Knockout 293T Cell Lysate

Product Discontinued

PI 3-Kinase p85 beta Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon 2
• Gene: PIK3R2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for PI 3-Kinase p85 beta Knockout 293T Cell Lysate

• P85B
• p85-beta
• phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
• phosphatidylinositol 3-kinase regulatory subunit beta
• phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta)
• phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• PI 3Kinase p85 beta
• PI 3-Kinase p85 beta
• PI3K regulatory subunit beta
• PI3-kinase regulatory subunit beta
• PI3-kinase subunit p85-beta
• PIK3R2
• ptdIns-3-kinase regulatory subunit beta
• ptdIns-3-kinase regulatory subunit p85-beta

Background

Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PIK3R2