PI 3-Kinase p85 beta Antibody (572001) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 572001
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

PI 3-Kinase p85 beta Antibody (572001) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human PI 3‑Kinase p85 beta
  Lys516-Gly642
  Accession # O00459
• Specificity: Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha , rhPI 3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIK3R2
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PI 3-Kinase p85 beta Antibody (572001) [CoraFluor™ 1]

• P85B
• p85-beta
• phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
• phosphatidylinositol 3-kinase regulatory subunit beta
• phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta)
• phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• PI 3Kinase p85 beta
• PI 3-Kinase p85 beta
• PI3K regulatory subunit beta
• PI3-kinase regulatory subunit beta
• PI3-kinase subunit p85-beta
• PIK3R2
• ptdIns-3-kinase regulatory subunit beta
• ptdIns-3-kinase regulatory subunit p85-beta

Background

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 beta). p85 beta is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 beta is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIK3R2