Phosphorylase B Antibody (2E9) - Azide and BSA Free

Western Blot: Phosphorylase B Antibody (2E9.) [H00005257-M02] - Detection against Immunogen (37.84 KDa) .

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Phosphorylase B Antibody (2E9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PHKB (NP_000284, 984 a.a. ~ 1093 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EDTLGNIDQPQYRQIVVELLMVVSIVLERNPELEFQDKVDLDRLVKEAFNEFQKDQSRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKAVMNLLLEGEVKPNNDDPCLIS
• Specificity: PHKB (2E9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHKB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Phosphorylase B Antibody (2E9) - Azide and BSA Free

• DKFZp781E15103
• FLJ41698
• phosphorylase b kinase regulatory subunit beta
• phosphorylase kinase beta-subunit
• Phosphorylase kinase subunit beta
• phosphorylase kinase, beta

Background

Phosphorylase B, also known by its gene name, PHKB, is an enzyme localized to the plasma membrane that catalyzes the phosphorylation of the amino acid residue Serine. Phosphorylase B has a 1,093 amino acid long isoform that is 125 kDa (the canonical sequence) and three other isoforms derived from alternative splicing. Current research on PHKB is being performed relating to several diseases and disorders including hepatitis and metabolic disorders, such as hypoglycemia and glycogen storage disease. Phosphorylase B has also been shown to have interactions with B Raf, hnRNP C1 + C2, PASK, and UBE3A in pathways such as Glucose and Glycogen Metabolism, PKA Signaling, cAMP Pathway, Insulin Signaling Pathway and Calcium Signaling Pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHKB
• Entrez:
  • 5257(Human)
• OMIM:
  • 172490(Human)
• Uniprot:
  • NP_000284(Human)