Phospholipase C delta 1 Antibody - BSA Free

Western Blot: Phospholipase C delta 1 Antibody [NBP2-19810] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/ Immunofluorescence: Phospholipase C delta 1 Antibody [NBP2-19810] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Phospholipase C delta 1 Antibody [NBP2-19810] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

• Reactivity: Hu, Po, Bv
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Phospholipase C delta 1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Phospholipase C delta 1. The exact sequence is proprietary.
• Predicted Species: Porcine (92%), Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLCD1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Phospholipase C delta 1 Antibody - BSA Free

• 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1
• EC 3.1.4.11
• Phosphoinositide phospholipase C-delta-1
• Phospholipase C delta 1
• phospholipase C, delta 1
• Phospholipase C-delta-1
• Phospholipase C-III
• PLCD1
• PLC-delta-1
• PLC-III

Background

Phosphoinositide-specific phospholipase C (PLC) acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. PLC comprises a diverse family of enzymes that differ in structure and tissue distribution (Berridge, 1993 [PubMed 8381210]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PLCD1
• Uniprot:
  • P51178(Human)