Phospholamban [p Ser16] Antibody - BSA Free

Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186] - Bovine cardiac preparation was treated with PKA or untreated. It was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospholamban. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.

• Reactivity: Hu, Bv
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Phospholamban [p Ser16] Antibody - BSA Free Summary

• Description: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Immunogen: Synthetic peptide corresponding to aa14-25, RA[pS]TIEMPQQAR-C of human cardiac phospholamban with a cysteine added at the C-terminal, conjugated to KLH. Species Sequence Homology: mouse, rat, rabbit, porcine and canine
• Modification: p Ser16
• Localization: Membrane
• Specificity: Recognizes bovine Phospho-lamban phosphorylated (Ser16) (5-25kD). Additional non-specific bands may be detected at 75 and 100kD in some lysates.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2 ug/ml
• Application Notes: Western Blot: 0.5-2ug/ml detects phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10ug PKA for 10 minutes. Bovine cardiac preparation was treated with PKA or untreated. It was resolved by electrophoresis, transferred to nitrocellulose and probed with NB600-1186(2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.

Reactivity Notes

Expected to cross-react with Mouse, Rat, Rabbit, Porcine and Canine due to sequence identity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH 7.4), 0.15M NaCl and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Alternate Names for Phospholamban [p Ser16] Antibody - BSA Free

• CMD1PPLBcardiac phospholamban
• phospholamban

Background

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (aa25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLN
• Entrez:
  • 5350(Human)
• Uniprot:
  • P26678(Human)