Phosphoglucomutase 5 Antibody (PGM5/3552)

Western Blot: Phosphoglucomutase 5 Antibody (PGM5/3552) [NBP3-13996] - Western Blot Analysis of human heart tissue lysate using Phosphoglucomutase 5 antibody (PGM5/3552).

Immunohistochemistry-Paraffin: Phosphoglucomutase 5 Antibody (PGM5/3552) [NBP3-13996] - Formalin-fixed, paraffin-embedded human uterus stained with Phosphoglucomutase 5 antibody (PGM5/3552)at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: Phosphoglucomutase 5 Antibody (PGM5/3552) [NBP3-13996] - Formalin-fixed, paraffin-embeddedhuman bladderstained with Phosphoglucomutase 5 antibody (PGM5/3552) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Protein Array: Phosphoglucomutase 5 Antibody (PGM5/3552) [NBP3-13996] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Phosphoglucomutase 5 Antibody (PGM5/3552).

• Reactivity: Hu, Mu, Rt, Bv, Rb
• Applications: WB, ELISA, ICC/IF, IHC, MA
• Clone: PGM5/3552
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Phosphoglucomutase 5 Antibody (PGM5/3552) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14132)
  
  Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human Phosphoglucomutase 5 protein (Uniprot: Q15124)
• Localization: Cytoplasm
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGM5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Porcine, Avian, and Frog.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Phosphoglucomutase 5 Antibody (PGM5/3552)

• aciculin
• EC 5.4.2.2
• PGM-RP
• PGMRPphosphoglucomutase-like protein 5
• phosphoglucomutase 5
• Phosphoglucomutase-related protein

Background

Phosphoglucomutases (EC 5.2.2.2.), such as PGM5, are phosphotransferases involved in interconversion of glucose-1-phosphate and glucose-6-phosphate. PGM activity is essential in formation of carbohydrates from glucose-6-phosphate and in formation of glucose-6-phosphate from galactose and glycogen (Edwards et al., 1995 [PubMed 8586438]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGM5
• Uniprot:
  • Q15124(Human)