PHGDH Antibody (4A3-1D6) - Azide and BSA Free

Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6. Analysis of PHGDH expression in human liver.

Immunocytochemistry/Immunofluorescence: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.

Immunohistochemistry-Paraffin: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.

Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6. Analysis of PHGDH expression in A-431.

Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6 Analysis of PHGDH expression in Jurkat.

Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody (M01), clone 4A3-1D6.Lane 1: PHGDH transfected lysate(56.7 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 1 ~ 10 ug/ml.

Immunoprecipitation: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH MaxPab rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 4A3-1D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PHGDH Antibody (4A3-1D6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PHGDH Antibody (4A3-1D6) - Azide and BSA Free (H00026227-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. Anti-PHGDH Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PHGDH (AAH11262.1, 1 a.a. ~ 533 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGILGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF
• Specificity: PHGDH - phosphoglycerate dehydrogenase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHGDH
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against recominant protein, tissue lysate and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PHGDH Antibody (4A3-1D6) - Azide and BSA Free

• 3,3-phosphoglycerate dehydrogenase
• 3PGDH
• 3-PGDH
• D-3-phosphoglycerate dehydrogenase
• EC 1.1.1
• EC 1.1.1.95
• MGC3017
• PDG
• PDGH3
• PGAD
• PGD
• PGDH
• PHGDH
• phosphoglycerate dehydrogenase
• SERA

Background

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHGDH Antibody (H00026227-M01)(2)

Publications using H00026227-M01

• Pollari S, Kakonen SM, Edgren H et al. Enhanced serine production by bone metastatic breast cancer cells stimulates osteoclastogenesis. Breast Cancer Res Treat. 2010-03-30 [PMID: 20352489]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: PHGDH
• Entrez:
  • 26227(Human)
• OMIM:
  • 601(Human)
• Uniprot:
  • AAH11262(Human)