PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free

Protein Array: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PHF10 Antibody (PCRP-PHF10-2A10).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free

Flow cytometric analysis of PFA-fixed HeLa cells. PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-PHF10-2A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13750).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length humanPHF10protein (Uniprot: Q8WUB8)
• Localization: Nucleus
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHF10
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order this antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 56.05 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Mouse and Chick.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free

• BAF45a
• BAF45APHD zinc finger protein XAP135
• BRG1-associated factor 45a
• FLJ10975
• MGC111009
• PHD finger protein 10
• XAP135BRG1-associated factor, 45-KD, A

Background

PHF10 contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHF10