PGRMC1 Antibody

Western Blot: PGRMC1 Antibody [NB100-2841] - Staining of Human Kidney (A), Liver (B) and Testes (C) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PGRMC1 Antibody [NB100-2841] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Tumorsphere: PGRMC1 Antibody [NB100-2841] - Staining of Mouse Testes lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PGRMC1 Antibody Summary

• Description: Novus Biologicals Goat PGRMC1 Antibody (NB100-2841) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-PGRMC1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EPKDESARKND corresponding to C-Terminus according to NP_006658.1.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PGRMC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: Western blot: Approx 24 kDa band observed in Human Kidney, Liver and Testis lysates, and approx 25 kDa in Mouse Testis lysates (calculated MW of 21.7kDa according to Human NP_006658.1 and Mouse NP_058063.2).
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Kidney Whole Tissue Lysate (Adult Whole Normal)
  PGRMC1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGRMC1 Antibody

• HPR6.6
• HPR6.6PGRMC
• membrane-associated progesterone receptor component 1
• MPR
• PGRMC1
• Progesterone Binding Protein
• progesterone receptor membrane component 1

Background

Progesterone binding protein is a putative steroid membrane receptor. The protein is expressed predominantly in the liver and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGRMC1 Antibody (NB100-2841)(2)

Publications using NB100-2841

• Engmann L, Losel R, Wehling M et al. Progesterone regulation of human granulosa/luteal cell viability by an RU486-independent mechanism. J Clin Endocrinol Metab 2006-12-01 [PMID: 16984987]
• Hand RA, Craven RJ. Hpr6.6 protein mediates cell death from oxidative damage in MCF-7 human breast cancer cells. J Cell Biochem 2003-10-15 [PMID: 14523988]

Bioinformatics

• Gene Symbol: PGRMC1
• Entrez:
  • 10857(Human)
  • 53328(Mouse)
• Uniprot:
  • O00264(Human)